Why must you use the same restriction enzyme on the cell dna and on the plasmid?
1 Answer
Nov 21, 2015
Restriction endonuclease identifies and cuts the same pallindromic sequence in both DNA and Vector due to which when they will be mixed , their complementary bases will join and it will form the r-DNA,
If both are cut with different RE , then on mixing they wont ligate with each other as their bases will not match.
