Western Blot

Key Questions

  • How much protein can a western blot detect?

    Answer:

    depends on various factors. I have detected 2#mu#g of protein.

    Explanation:

    there are various factors which are involved in the process which include.

    1. amount of protein in cell lysate
    2. effectiveness/sensitivity of the antibodies
    3. substrate sensitivity used for chemiluminescence

    i have detected about 2#mu#g of a membrane protein in my cell lysate.

    with purified protein i could detect approximate 1#mu#g.

    but above three factors are crucial.

    hsk · 1 · Nov 13 2015

Questions

Genetic Engineering