What is cDNA?

1 Answer
Jan 3, 2018

It is a DNA molecule synthesized from template mRNA in reverse transcription

Explanation:

cDNA or complementary DNA has nucleotide sequence complementary to the processed mRNA from which it has been synthesized in a process called reverse transcription that is catalyzed by enzyme i.e reverse transcriptase.

cDNA being synthesized from single stranded mRNA is firstly synthesized as a single stranded molecule. And it remains single stranded until PCR(polymerase chain reaction) is carried out. In PCR that is aided by DNA polymerase (e.g Taq DNA polymerase) , it gets it's double stranded form.

http://evrogen.com/technologies/Mint-cDNA-synthesis.shtmlhttp://evrogen.com/technologies/Mint-cDNA-synthesis.shtml

Hope it helps...